The present study was designed to search for compounds with analgesic activity from the Schizophyllum commune (SC), which is widely consumed as edible and medicinal mushroom world. Thin layer chromatography (TLC), tosilica gel column chromatography, sephadex LH 20, and reverse-phase high performance liquid chromatography (RP-HPLC) were used to isolate and purify compounds from SC. Structural analysis of the isolated compounds was based on nuclear magnetic resonance (NMR). The effects of these compounds on voltage-gated sodium (NaV) channels were evaluated using patch clamp. The analgesic activity of these compounds was tested in two types of mouse pain models induced by noxious chemicals. Five phenolic acids identified from SC extracts in the present study included vanillic acid, m-hydroxybenzoic acid, o-hydroxybenzeneacetic acid, 3-hydroxy-5-methybenzoic acid, and p-hydroxybenzoic acid. They inhibited the activity of both tetrodotoxin-resistant (TTX-r) and tetrodotoxin-sensitive (TTX-s) NaV channels. All the compounds showed low selectivity on NaV channel subtypes. After intraperitoneal injection, three compounds of these compounds exerted analgesic activity in mice. In conclusion, phenolic acids identified in SC demonstrated analgesic activity, facilitating the mechanistic studies of SC in the treatment of neurasthenia.
Analgesics/administration & dosage , Hydroxybenzoates/administration & dosage , Neurasthenia/drug therapy , Schizophyllum/chemistry , Voltage-Gated Sodium Channel Blockers/administration & dosage , Voltage-Gated Sodium Channels/metabolism , Analgesics/chemistry , Analgesics/isolation & purification , Animals , Humans , Hydroxybenzoates/chemistry , Hydroxybenzoates/isolation & purification , Mice , Neurasthenia/genetics , Neurasthenia/metabolism , Voltage-Gated Sodium Channel Blockers/chemistry , Voltage-Gated Sodium Channel Blockers/isolation & purification , Voltage-Gated Sodium Channels/genetics
The data on cytogenetic examination concerning the offspring of the Chernobyl accident liquidators (cleanup personnel) have been obtained. It has been established that spontaneous chromosomal aberrations level before folic acid administration was 1,8 times higher than that value after its employment (4,45 to 2,42 %, p < 0,01). In lymphocyte cultures treated with mitomycin C accompanied by folic acid it was 4,5 times higher before their administration (23,95 to 5,36 %, p < 0,001). The data obtained confirm a possibility of stabilization of genetic apparatus in offspring of the Chernobyl disaster liquidators after folic acid administration.
Chernobyl Nuclear Accident , Chromosome Aberrations , Folic Acid/pharmacology , Leukocytes, Mononuclear/pathology , Military Personnel , Occupational Exposure , Adolescent , Cells, Cultured , Child , Chromosome Aberrations/drug effects , Chronic Disease , Cytogenetic Analysis , Female , Folic Acid/therapeutic use , Gastrointestinal Diseases/genetics , Gastrointestinal Diseases/prevention & control , Humans , Male , Mitomycin/pharmacology , Neurasthenia/genetics , Neurasthenia/prevention & control , Paternal Exposure
Neurasthenia/history , Cultural Characteristics , Electrophysiology/history , Germany , Health Resources/history , History, 19th Century , History, 20th Century , Humans , Nervous System Physiological Phenomena , Neurasthenia/genetics , Psychiatry/history , Social Values , Sociology, Medical/history
BACKGROUND: Somatoform disorders such as neurasthenia and chronic fatigue syndrome are characterized by a combination of prolonged mental and physical fatigue. This study aimed to investigate the heritability of somatic distress and determine whether this dimension is aetiologically distinct from measures of depression and anxiety. METHOD: Measures of anxiety, depression, phobic anxiety, somatic distress and sleep difficulty were administered in a self-report questionnaire to a community-based sample of 3469 Australian twin individuals aged 18 to 28 years. Factor analysis using a Promax rotation, produced four factors: depression, phobic anxiety, somatic distress and sleep disturbance. Multivariate and univariate genetic analyses of the raw categorical data scores for depression, phobic anxiety and depression were then analysed in Mx1.47. RESULTS: Univariate genetic analysis revealed that an additive genetic and non-shared environmental (AE) model best explained individual differences in depression and phobic anxiety scores, for male and female twins alike, but could not resolve whether additive genes or shared environment were responsible for significant familial aggregation in somatic distress. However, multivariate genetic analysis showed that an additive genetic and non-shared environment (AE) model best explained the covariation between the three factors. Furthermore, 33 % of the genetic variance in somatic distress was due to specific gene action unrelated to depression or phobic anxiety. In addition, 74% of the individual environmental influence on somatic distress was also unrelated to depression or phobic anxiety. CONCLUSION: These results support previous findings that somatic symptoms are relatively aetiologically distinct both genetically and environmentally from symptoms of anxiety and depression.
Arousal/genetics , Diseases in Twins , Somatoform Disorders/genetics , Adolescent , Adult , Australia , Depressive Disorder/genetics , Depressive Disorder/psychology , Fatigue Syndrome, Chronic/genetics , Fatigue Syndrome, Chronic/psychology , Female , Genetic Predisposition to Disease/genetics , Humans , Male , Models, Genetic , Neurasthenia/genetics , Neurasthenia/psychology , Phobic Disorders/genetics , Phobic Disorders/psychology , Risk Factors , Social Environment , Somatoform Disorders/psychology
The authors studied the family history of 103 patients with slowly progressive schizophrenia (1218 relatives) characterized by the predominance of obsessions and hysterical disturbances. The study disclosed the accumulation of both various forms of schizophrenia and personality anomalies of psychoasthenic and hysterical types among the relatives of the patients. The clinical specific features of "linear" symptomatology in the families studied were considered. The role of one or another type of constitutional predisposition in the development of phenotypical variants of the disease is shown.
Schizophrenia/genetics , Adolescent , Adult , Bipolar Disorder/genetics , Female , Humans , Hysteria/genetics , Male , Middle Aged , Neurasthenia/genetics , Obsessive Behavior , Personality Disorders/genetics , Phenotype , Risk
Schizophrenia, Paranoid/genetics , Adult , Cyclothymic Disorder/genetics , Depressive Disorder, Major/genetics , Epilepsy/genetics , Female , Heart Defects, Congenital/complications , Heart Defects, Congenital/genetics , Histrionic Personality Disorder/genetics , Humans , Hypertension/complications , Hypertension/genetics , Intracranial Arteriosclerosis/complications , Intracranial Arteriosclerosis/genetics , Male , Myocardial Infarction/complications , Myocardial Infarction/genetics , Neurasthenia/genetics , Rheumatic Diseases/complications , Rheumatic Diseases/genetics , Schizoid Personality Disorder/genetics , Schizophrenia, Paranoid/complications , Sex Factors
Neurasthenia , Europe , Fatigue , Humans , Neurasthenia/chemically induced , Neurasthenia/classification , Neurasthenia/diagnosis , Neurasthenia/etiology , Neurasthenia/genetics , Neurasthenia/metabolism , Neurasthenia/therapy , Psychophysiologic Disorders/etiology , Psychotherapy , United States , World Health Organization
